RT Journal Article SR Electronic T1 Differential Proteomic Analysis of Hepatocellular Carcinomas from Ppp2r5d Knockout Mice and Normal (Knockout) Livers JF Cancer Genomics - Proteomics JO Cancer Genomics Proteomics FD International Institute of Anticancer Research SP 669 OP 685 DO 10.21873/cgp.20222 VO 17 IS 6 A1 CAROLINE LAMBRECHT A1 GABRIELA BOMFIM FERREIRA A1 JUDIT DOMÈNECH OMELLA A1 LOUIS LIBBRECHT A1 RITA DE VOS A1 RITA DERUA A1 CHANTAL MATHIEU A1 LUT OVERBERGH A1 ETIENNE WAELKENS A1 VEERLE JANSSENS YR 2020 UL http://cgp.iiarjournals.org/content/17/6/669.abstract AB Background: Hepatocellular carcinoma (HCC) is the major type of primary liver cancer. Mice lacking the tumor-suppressive protein phosphatase 2A subunit B56δ (Ppp2r5d) spontaneously develop HCC, correlating with increased c-MYC oncogenicity. Materials and Methods: We used two-dimensional difference gel electrophoresis-coupled matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify differential proteomes of livers from wild-type, non-cancerous and HCC-affected B56δ knockout mice. Results: A total of 23 proteins were differentially expressed/regulated in liver between wild-type and non-cancerous knockout mice, and 119 between non-cancerous and HCC knockout mice (‘cancer proteins’). Overlap with our reported differential transcriptome data was poor. Overall, 56% of cancer proteins were reported before in HCC proteomics studies; 44% were novel. Gene Ontology analysis revealed cancer proteins mainly associated with liver metabolism (18%) and mitochondria (15%). Ingenuity Pathway Analysis identified ‘cancer’ and ‘gastrointestinal disease’ as top hits. Conclusion: We identified several proteins for further exploration as novel potential HCC biomarkers, and independently underscored the relevance of Ppp2r5d knockout mice as a valuable hepatocarcinogenesis model.