PT - JOURNAL ARTICLE AU - MIGUEL A. FERNÁNDEZ-ROJAS AU - JORGE MELENDEZ-ZAJGLA AU - VILMA MALDONADO LAGUNAS TI - lincRNA-RP11400K9.4 Regulates Cell Survival and Migration of Breast Cancer Cells AID - 10.21873/cgp.20231 DP - 2020 Nov 01 TA - Cancer Genomics - Proteomics PG - 769--779 VI - 17 IP - 6 4099 - http://cgp.iiarjournals.org/content/17/6/769.short 4100 - http://cgp.iiarjournals.org/content/17/6/769.full SO - Cancer Genomics Proteomics2020 Nov 01; 17 AB - Background/Aim: Several works in the past decades pointed out the key role of long intergenic non-coding RNA (lincRNA) in breast cancer development. Here in we report for first time the importance of deregulation of lincRNA RP11-400K9.4 in breast cancer cells which played a role in cell survival and migration. Materials and Methods: After RP11-400K9.4 silencing by short hairpin RNAs or overexpression by GeneBlocks, real-time quantitative polymerase chain reaction (RT-PCR), microarray, migration, proliferation and viability assay were performed. Results: RP11-400K9.4 expression was mainly in the cytoplasmic fraction in 2D culture. Overexpression of RP11-400K9.4 led to a reduction of migration by MCF-7 and MDA-MB-368 cells and an increase in cellular survival after UV-C radiation. Bioinformatic analyses highlighted irradiation-induced DNA damage, DNA repair and cell-cycle pathways as the mainly affected by RP11-400K9.4. Furthermore RT-PCR assay demonstrated the overexpression of baculoviral IAP repeat containing 3 (BIRC3) a known oncogene that promotes radiotherapy resistance through the nuclear factor kappa B (NFĸB) pathway. Conclusion: RP11-400K9.4 participates in the modulation of migration and survival processes probably via the BIRC3/NFĸB pathway.