RT Journal Article SR Electronic T1 Normal Mouse Brain Proteome II: Analysis of Brain Regions by High-resolution Mass Spectrometry JF Cancer Genomics - Proteomics JO Cancer Genomics Proteomics FD International Institute of Anticancer Research SP 757 OP 767 DO 10.21873/cgp.20230 VO 17 IS 6 A1 ARTEMIS G. KOROVESI A1 ATHANASIOS K. ANAGNOSTOPOULOS A1 VASILEIOS PIERROS A1 DIMITRIOS J. STRAVOPODIS A1 GEORGE TH. TSANGARIS YR 2020 UL http://cgp.iiarjournals.org/content/17/6/757.abstract AB Background/Aim: Proteomics technologies provide fundamental insights into the high organizational complexity and diversity of the central nervous system. In the present study, high-resolution mass spectrometry (MS) was applied in order to identify whole-proteome content of anatomically distinct and functionally specific mouse brain regions. Materials and Methods: Brains from eight 8-week-old C57BL/6N normal male mice were separated into seven anatomically district regions. The protein content of each region was analyzed by high-throughput nano-liquid chromatography-MS/MS Orbitrap elite technology. Results: A total of 16,574 proteins were identified: 2,795 in cerebral cortex, 2,311 in olfactory bulb, 2,246 in hippocampus, 2,247 in hypothalamus, 2,250 in mid brain, 2,334 in cerebellum and 2,391 in medulla. Of these proteins, 534 were uniquely expressed in cerebral cortex, 323 in olfactory bulb, 230 in hippocampus, 272 in hypothalamus, 1,326 in mid brain, 320 in cerebellum and 268 in medulla. Conclusion: These data represent the most comprehensive proteomic map of the normal mouse brain and they might further be used in studies related to brain diseases, including cancer and neurodegenerative diseases.