TY - JOUR T1 - NONO Is a Negative Regulator of <em>SOX2</em> Promoter JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 359 LP - 367 DO - 10.21873/cgp.20195 VL - 17 IS - 4 AU - SHANSHAN LIANG AU - HIDEHISA TAKAHASHI AU - TETSURO HIROSE AU - YASUHIRO KURAMITSU AU - SHIGETSUGU HATAKEYAMA AU - HIRONORI YOSHIYAMA AU - RUOYU WANG AU - JUN-ICHI HAMADA AU - HISASHI IIZASA Y1 - 2020/07/01 UR - http://cgp.iiarjournals.org/content/17/4/359.abstract N2 - Background/Aim: Sex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown. Materials and Methods: SOX2 promoter-binding proteins were identified by liquid chromatography–mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments. Results: Non-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain. Conclusion: These results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells. ER -