PT  - JOURNAL ARTICLE
AU  - SHANSHAN LIANG
AU  - HIDEHISA TAKAHASHI
AU  - TETSURO HIROSE
AU  - YASUHIRO KURAMITSU
AU  - SHIGETSUGU HATAKEYAMA
AU  - HIRONORI YOSHIYAMA
AU  - RUOYU WANG
AU  - JUN-ICHI HAMADA
AU  - HISASHI IIZASA
TI  - NONO Is a Negative Regulator of <em>SOX2</em> Promoter
AID  - 10.21873/cgp.20195
DP  - 2020 Jul 01
TA  - Cancer Genomics - Proteomics
PG  - 359--367
VI  - 17
IP  - 4
4099  - http://cgp.iiarjournals.org/content/17/4/359.short
4100  - http://cgp.iiarjournals.org/content/17/4/359.full
SO  - Cancer Genomics Proteomics2020 Jul 01; 17
AB  - Background/Aim: Sex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown. Materials and Methods: SOX2 promoter-binding proteins were identified by liquid chromatography–mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments. Results: Non-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain. Conclusion: These results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells.