TY - JOUR T1 - TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140 JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 23 LP - 33 DO - 10.21873/cgp.20164 VL - 17 IS - 1 AU - GUANGWEI ZHU AU - CHUNLIN LIN AU - ZHIBIN CHENG AU - QIN WANG AU - ROBERT M. HOFFMAN AU - SHREE RAM SINGH AU - YONGJIAN HUANG AU - WEI ZHENG AU - SHUGANG YANG AU - JIANXIN YE Y1 - 2020/01/01 UR - http://cgp.iiarjournals.org/content/17/1/23.abstract N2 - Background/Aim: Colorectal cancer (CRC) cells secrete inflammatory cytokines that affect CRC progression. The aim of the present study was to determine if micro-RNA-140(miR-140) regulates inflammatory cytokine secretion induced by lipopolysaccharide (LPS) in colorectal cancer cells by targeting tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). Materials and Methods: Fifty fresh colon-cancer specimens and normal colorectal tissues were collected from patients with CRC and tested for the expression miR-140. Human CRC cell lines SW480 and HCT116 were treated with various concentrations and times with LPS. miR-140 and mRNA expression of potentially related genes were analyzed by qPCR. Protein expression was analyzed using western blot or ELISA. Overexpression plasmids with pcDNA3.1-TRAF6, pGL4.10-wtTRAF6 and pGL4.10-mutTRAF6 were constructed. miRNA target gene prediction and a dual luciferase assay were used to analyze miR-140-targeted TRAF6. Results: miR-140 expression was up-regulated in CRC tissues. In CRC cells, LPS could increase miR-140 expression in a time- and concentration-dependent manner. LPS increased inflammatory cytokine mRNA expression levels in SW480 and HCT116 human colon-cancer cells. miRNA-140 suppressed TRAF6 expression via targeting the 3′UTR. TRAF6 affected miR-140-mediated inflammatory cytokine expression of SW480 and HCT116 cells under LPS treatment. Conclusion: miR-140 regulates inflammatory cytokine secretion of LPS-induced colorectal cancer cells by targeting TRAF6. ER -