RT Journal Article
SR Electronic
T1 Co-culture With Human Breast Adipocytes Differentially Regulates Protein Abundance in Breast Cancer Cells
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 319
OP 332
DO 10.21873/cgp.20137
VO 16
IS 5
A1 REBEKAH LEE ISLA CRAKE
A1 ELISABETH PHILLIPS
A1 TORSTEN KLEFFMANN
A1 MARGARET JANE CURRIE
YR 2019
UL http://cgp.iiarjournals.org/content/16/5/319.abstract
AB Background/Aim: Recent research highlights the role of cancer-associated adipocytes (CAA) in promoting breast cancer cell migration, invasion and resistance to therapy. This study aimed at identifying cellular proteins differentially regulated in breast cancer cells co-cultured with CAA. Materials and Methods: Adipocytes isolated from human breast adipose tissue were co-cultured with hormone receptor-positive (MCF-7) or -negative (MDA-MB-231) breast cancer cells using a transwell co-culture system. Proteomes of co-cultured and control breast cancer cells were compared quantitatively using iTRAQ labelling and tandem mass spectrometry, and the results were validated by western blotting. Results: A total of 1,126 and 1,218 proteins were identified in MCF-7 and MDA-MB-231 cells, respectively. Among these, 85 (MCF-7) and 63 (MDA-MB-231) had an average fold change >1.5 following co-culture. Pathway analysis revealed that CAA-induced enrichment of proteins involved in metabolism, the ubiquitin proteasome, and purine synthesis. Conclusion: This study provides a proteomic platform for investigating the paracrine role of CAA in promoting breast cancer cell metastasis and resistance to therapy.