PT - JOURNAL ARTICLE AU - MANPREET S. CHAHAL AU - H. TERESA KU AU - ZHIHONG ZHANG AU - CHRISTIAN M. LEGASPI AU - ANGELA LUO AU - MANDI M. HOPKINS AU - KATHRYN E. MEIER TI - Differential Expression of <em>Ccn4</em> and Other Genes Between Metastatic and Non-metastatic EL4 Mouse Lymphoma Cells DP - 2016 Nov 01 TA - Cancer Genomics - Proteomics PG - 437--442 VI - 13 IP - 6 4099 - http://cgp.iiarjournals.org/content/13/6/437.short 4100 - http://cgp.iiarjournals.org/content/13/6/437.full SO - Cancer Genomics Proteomics2016 Nov 01; 13 AB - Background: Previous work characterized variants of the EL4 murine lymphoma cell line. Some are non-metastatic, and others metastatic, in syngenic mice. In addition, metastatic EL4 cells were stably transfected with phospholipase D2 (PLD2), which further enhanced metastasis. Materials and Methods: Microarray analyses of mRNA expression was performed for non-metastatic, metastatic, and PLD2-expressing metastatic EL4 cells. Results: Many differences were observed between non-metastatic and metastatic cell lines. One of the most striking new findings was up-regulation of mRNA for the matricellular protein WNT1-inducible signaling pathway protein 1 (CCN4) in metastatic cells; increased protein expression was verified by immunoblotting and immunocytochemistry. Other differentially expressed genes included those for reproductive homeobox 5 (Rhox5; increased in metastatic) and cystatin 7 (Cst7; decreased in metastatic). Differences between PLD2-expressing and parental cell lines were limited but included the signaling proteins Ras guanyl releasing protein 1 (RGS18; increased with PLD2) and suppressor of cytokine signaling 2 (SOCS2; decreased with PLD2). Conclusion: The results provide insights into signaling pathways potentially involved in conferring metastatic ability on lymphoma cells.