TY - JOUR T1 - Characterization of Heparan Sulfate Proteoglycan-positive Recycling Endosomes Isolated from Glioma Cells JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 443 LP - 452 VL - 13 IS - 6 AU - KATARZYNA A. PODYMA-INOUE AU - TAKUYA MORIWAKI AU - ANUPAMA R. RAJAPAKSHE AU - KAZUE TERASAWA AU - MIKI HARA-YOKOYAMA Y1 - 2016/11/01 UR - http://cgp.iiarjournals.org/content/13/6/443.abstract N2 - Background: Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. Materials and Methods: Subcellular fractionation was used to separate vesicles containing HSPGs from the rat C6 glioma cell line. Isolated HSPG-positive vesicles were further characterized with liquid chromatography-mass spectrometry. Results: The HSPG-positive vesicular fractions, distinct from plasma membrane-derived material, were enriched in endocytic marker, Rab11. Proteomic analysis identified more than two hundred proteins to be associated with vesicular membrane, among them, over eighty were related to endosomal uptake, recycling or vesicular transport. Conclusion: Part of HSPGs in glioma cells is internalized through clathrin-dependent endocytosis and undergo recycling. The development of compounds regulating HSPG-mediated trafficking will likely enable design of effective glioma treatment. ER -