RT Journal Article
SR Electronic
T1 Genome-wide microRNA Expression Profiling in Primary Tumors and Matched Liver Metastasis of Patients with Colorectal Cancer
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 311
OP 316
VO 13
IS 4
A1 PETRA VYCHYTILOVA-FALTEJSKOVA
A1 MARTIN PESTA
A1 LENKA RADOVA
A1 VACLAV LISKA
A1 ONDREJ DAUM
A1 ZDENEK KALA
A1 MAREK SVOBODA
A1 IGOR KISS
A1 ONDREJ SLABY
YR 2016
UL http://cgp.iiarjournals.org/content/13/4/311.abstract
AB Background: Primary tumor spread to the liver is the major cause of disease progression and death in patients with colorectal cancer (CRC). MicroRNAs (miRNAs) are small non-coding RNAs that are involved in cancer development and progression, but their role in metastasis has not been extensively investigated. Materials and Methods: Firstly, expression profiling of 752 miRNAs in 20 primary tumors and their corresponding liver metastases was performed. Secondly, validation of the results was carried out on an independent cohort of 66 patients with metastatic CRC using reverse transcription–quantitative polymerase chain (RT-qPCR) reaction. Results: In total, 33 miRNAs were found to be significantly deregulated in liver metastases compared to their primary tumors. Fifteen miRNAs were chosen for subsequent validation, which confirmed significantly reduced expression of miR-143, miR 10b, and miR-28-5p, and increased expression of miR 122, miR-122*, and miR 885-5p in the tissue of liver metastases. Conclusion: These results indicate that miRNAs could serve as new therapeutic targets in patients with metastatic CRC.