TY - JOUR T1 - Expression of microRNAs of C19MC in Different Histological Types of Testicular Germ Cell Tumour JF - Cancer Genomics - Proteomics JO - Cancer Genomics Proteomics SP - 281 LP - 289 VL - 13 IS - 4 AU - INGA FLOR AU - MEIKE SPIEKERMANN AU - THOMAS LÖNING AU - KLAUS-PETER DIECKMANN AU - GAZANFER BELGE AU - JÖRN BULLERDIEK Y1 - 2016/07/01 UR - http://cgp.iiarjournals.org/content/13/4/281.abstract N2 - Background: Testicular germ cell tumours (TGCTs) are the most common tumours in men aged from 20 to 40 years, with a steadily increasing incidence. This study aimed to characterize the expression of the miRNA cluster C19MC in TGCT and to evaluate the suitability of a C19MC miRNA as a serum biomarker. Materials and Methods: By quantitative reverse transcription PCR, we measured the expression of miR-517a-3p, miR-519a-3p, and miR-519c 3p in tissue samples of 25 TGCTs and the level of miR-517a-3p in serum samples obtained pre- and postoperatively from the same patients. Results: We detected a significantly higher expression of C19MC miRNAs in non-seminomas than in seminomas and in clinical stages 2 and 3 than in stage 1 in both tissue and serum samples. Conclusion: miRNAs of C19MC are overexpressed in more aggressive types of TGCT, suggesting they contribute to malignancy. Furthermore, they might serve as serum biomarkers for these types of TGCT. ER -