@article {ZHENG231, author = {HUILI ZHENG and YAN WANG and CHUANNING TANG and LINDSEY JONES and HUA YE and GUANGCHUN ZHANG and WEIHAI CAO and JINGWEN LI and LIFENG LIU and ZHENCONG LIU and CHAO ZHANG and FENG LOU and ZHIYUAN LIU and YANGYANG LI and ZHENFEN SHI and JINGBO ZHANG and DANDAN ZHANG and HONG SUN and HAICHAO DONG and ZHISHOU DONG and BAISHUAI GUO and HE YAN and QINGYU LU and XUE HUANG and SI-YI CHEN}, title = {TP53, PIK3CA, FBXW7 and KRAS Mutations in Esophageal Cancer Identified by Targeted Sequencing}, volume = {13}, number = {3}, pages = {231--238}, year = {2016}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Esophageal cancer (EC) is a common malignancy with significant morbidity and mortality. As individual cancers exhibit unique mutation patterns, identifying and characterizing gene mutations in EC that may serve as biomarkers might help predict patient outcome and guide treatment. Traditionally, personalized cancer DNA sequencing was impractical and expensive. Recent technological advancements have made targeted DNA sequencing more cost- and time-effective with reliable results. This technology may be useful for clinicians to direct patient treatment. Materials and Methods: The Ion PGM and AmpliSeq Cancer Panel was used to identify mutations at 737 hotspot loci of 45 cancer-related genes in 64 EC samples from Chinese patients. Results: Frequent mutations were found in TP53 and less frequent mutations in PIK3CA, FBXW7 and KRAS. Conclusion: These results demonstrate that targeted sequencing can reliably identify mutations in individual tumors that make this technology a possibility for clinical use.}, issn = {1109-6535}, URL = {https://cgp.iiarjournals.org/content/13/3/231}, eprint = {https://cgp.iiarjournals.org/content/13/3/231.full.pdf}, journal = {Cancer Genomics \& Proteomics} }