PT  - JOURNAL ARTICLE
AU  - RICHARD I. SOMIARI
AU  - REBECCA SUTPHEN
AU  - KUTRALANATHAN RENGANATHAN
AU  - STEPHEN RUSSELL
AU  - ELZBIETA PASTWA
AU  - STELLA A. SOMIARI
TI  - A Low-density Antigen Array for Detection of Disease-associated Autoantibodies in Human Plasma
DP  - 2016 Jan 01
TA  - Cancer Genomics - Proteomics
PG  - 13--19
VI  - 13
IP  - 1
4099  - http://cgp.iiarjournals.org/content/13/1/13.short
4100  - http://cgp.iiarjournals.org/content/13/1/13.full
SO  - Cancer Genomics Proteomics2016 Jan 01; 13
AB  - Background/Aim: The ability to easily detect autoantibodies will help in the early diagnosis and treatment of certain diseases. Currently, available methods for autoantibody detection are time-consuming and cumbersome. The present study aimed to evaluate the performance of an easy-to-use antigen array developed for autoantibody detection. Materials and Methods: Plasma from 9 female donors diagnosed with ovarian cancer (test group) and 9 matched donors with no history of cancer (reference group) were screened and results were compared. Autoantibody levels ≥1.5-times the background were classified as positive. Results: A total of 29 autoantibodies were detected, out of which the autoantibody against osteoprotegerin was found to be significantly higher in the “test” group (p<0.001) while those against macrophage migration inhibitor factor, interleukin-2 and vascular endothelial growth factor were lower (p<0.05). Conclusion: The evaluated antigen array has potential as a simple method for determining the presence/absence of up to 90 disease-associated autoantibodies in a plasma specimen.