RT Journal Article
SR Electronic
T1 Association Between <em>RASSF1A</em> Promoter Methylation and Testicular Germ Cell Tumor: A Meta-analysis and a Cohort Study
JF Cancer Genomics - Proteomics
JO Cancer Genomics Proteomics
FD International Institute of Anticancer Research
SP 363
OP 372
VO 14
IS 5
A1 DORA MARKULIN
A1 ALEKSANDAR VOJTA
A1 IVANA SAMARŽIJA
A1 MARIJA GAMULIN
A1 IVONA BEČEHELI
A1 IRENA JUKIĆ
A1 ČEDOMIR MAGLOV
A1 VLATKA ZOLDOŠ
A1 ALEKSANDRA FUČIĆ
YR 2017
UL http://cgp.iiarjournals.org/content/14/5/363.abstract
AB Background: The RAS association domain family protein 1a (RASSF1A) is a prominent tumor suppressor gene showing altered promoter methylation in testicular germ cell tumors (TGCT). RASSF1A promoter hypermethylation might represent an early event in TGCT tumorigenesis. We investigated whether the RASSF1A promoter methylation in peripheral blood of TGCT patients can be associated with testicular cancer risk. Materials and Methods: Following a meta-analysis, we performed a cohort study including 32 testicular cancer patients and 32 healthy controls. Promoter methylation of the RASSF1A and O6-methylguanine-DNA-methyltransferase (MGMT) genes was analyzed using bisulfite pyrosequencing of DNA from peripheral blood. Results: Meta-analysis showed an odds ratio (OR) of 7.69 for RASSF1A promoter methylation as a risk factor for TGCT. Cohort study found altered methylation of the RASSF1A promoter in blood of TGCT patients. Methylation was higher in TGCT patients before BEP chemotherapy. Conclusion: The meta-analysis indicates a role of the RASSF1A promoter hypermethylation from peripheral blood in TCGT. We confirmed that finding in our cohort study, which represents the first report of changed RASSF1A promoter methylation in peripheral blood TGCT.