Figure 2.
A: Deletion analysis of chromosome 6 and 6q transferred into rat and human tumor cell lines. Partial chromosome 6 cytogenetic bands are shown with the interval q16.3 expanded. The polymorphic markers designated in the first column were amplified by PCR using the DNA from specific clones as template. RA6A and RA6q cell lines were used as source of donor chromosome. The presence of all markers in RA6A is shown, and these markers were also present in RA6q (not shown in the figure). ROSE199, ROVE12, NMU, C6 and SKOV3 are recipient cell lines. Suffixes R1, R2, R3 and R4 indicate the revertant clones. ROSE/6M1, ROV/6R1, ROV/6R2 and ROV/6R3 represent mixed cell populations of revertant clones. Filled rectangles indicate the absence of a specific marker, empty rectangles designate the presence of a specific marker and X indicates a noninformative marker. The noninformative markers were nonpolymorphic between donor chromosome and SKOV3 cells. B: Physical map of 6q16.3. The location of DNA markers, corresponding YAC and BAC clones spanning the SEN6A locus are indicated.