Article Figures & Data
Figures
- Figure 1.
A: CL-A cells shortly after transformation, stained for alkaline phosphatase. The arrow indicates normal osteoblast-like cells of the culture next to transformed cells on the right (magnification: ×100). B: Clonogenic growth of CL-A cells (unstained, magnification: ×200). C: CL-B cells stained for calcification with Alizarin red (magnification: ×100).
- Figure 2.
Growth of normal osteoblast-like cells and of cell lines CL-A and CL-B in cell culture medium supplemented with 10 % FCS (A) or 0.5 % FCS (B). Five-hundred cells were seeded in the wells of a 96-well culture plate. After 4 h of adhesion (day 0) and at indicated intervals, lysosomal hexosaminidase activity was determined by a colorimetric assay according to Landegren (8), where absorbance at 405 nm is proportional to the number of cells. Experiments were performed three times in triplicate; mean values plus standard deviation are shown. ▪ Normal osteoblast-like cells; ▴ CL-A; ♦ CL-B.
- Figure 3.
Anchorage-independent growth of normal osteoblast-like cells of patient A and transformed cells CL-A in soft agarose. A total of 10,000 normal osteoblast-like cells, CL-A, and Saos-2 cells were grown for 2 weeks in 0.3% soft agarose, fixed with ethanol, and stained with 0.05% crystal violet. A: Normal osteoblast-like cells of patient A before spontaneous transformation. B: Osteoblast-like cells (CL-A) of patient A after spontaneous transformation. C: Saos-2 control cells.
- Figure 4.
G-banded karyotype of cell lines CL-A and CL-B.
