Figure 4.
Bioinformatics analysis of proteins identified by LC/LC/MS/MS analysis. A, Classification of 252 proteins within selected Gene Ontology (GO) terms for biological processes and cellular components. B, Functional enrichment analysis of celecoxib-modulated proteins by Ingenuity Pathway Analysis Tool. The functional terms 1-12 were identified for their significant enrichment levels using p-values calculated with right-tailed fisher's exact test. The threshold line in the graph corresponds to a p-value of 0. 05 as conventional cut-off. The functional terms above the threshold line would be considered as significantly modulated by celecoxib. 1: cardiovascular system development, 2: hematological system development, 3: cardiovascular disease, 4: hematological disease, 5: cell cycle, 6: cancer, 7: cell death, 8: immune response, 9: immune and lymphatic system development, 10: immunological disease, 11: tumor morphology, 12: inflammatory disease. C, Network view of associated proteins created within Ingenuity Pathway Analysis Tool (modified for clarity) with some of the celecoxib-modulated proteins. Red nodes: proteins up-regulated by celecoxib, green nodes: proteins down-regulated by celecoxib; white nodes: proteins not in celecoxib-modulated list, but with evidence of association with celecoxib-modulated proteins in the network annotated in Ingenuity database.