The Proteome Profile of the Human Osteosarcome Saos2 Cell Line

Abstract

The human Saos2 cell line was one of the first generated cell lines and is known to be used in all sorts of biomedical research. Knowledge of its protein expression is limited and no comprehensive study on the proteome of this cell type has been reported to date. Proteomics technologies were applied in order to analyse the proteins of the Saos2 cell line. Total protein extracts were separated by two dimensional gel electrophoresis (2-DE) and analysed by MALDI-MS and MALDI-MS-MS following in-gel digestion with trypsin and, finally, protein identification was carried out by peptide mass fingerprint (PMF) and post source decay (PSD), respectively. Approximately 4,000 spots were excised from four 2-DE gels and were analysed, resulting in the identification of 349 different gene products. The majority of the identified proteins were enzymes, regulatory proteins and transporters, while leukocyte markers and oncogenes were also included. Our findings include 10 protooncogenes (STRAP, FUBP1, SRC8, NPM, PARK7, DJ-1, PSD10, OXRP, GAGD2 and TPD54) related to the chromosomally instable character observed in the Saos2 cell line. Considering these, the Saos2 2-DE database shapes the basis for future expressional studies at the protein level and it forms a useful tool in anticancer research.