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Research Article

Transient Expression of a Major Ampullate Spidroin 1 Gene Fragment from Euprosthenops sp. in Mammalian Cells

STEFAN GRIP, ANNA RISING, HELENA NIMMERVOLL, ERICA STORCKENFELDT, SIMON J. MCQUEEN-MASON, NATALIA POUCHKINA-STANTCHEVA, FRITZ VOLLRATH, WILHELM ENGSTRÖM and ARMANDO FERNANDEZ-ARIAS
Cancer Genomics & Proteomics March 2006, 3 (2) 83-87;
STEFAN GRIP
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ANNA RISING
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HELENA NIMMERVOLL
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ERICA STORCKENFELDT
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SIMON J. MCQUEEN-MASON
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NATALIA POUCHKINA-STANTCHEVA
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FRITZ VOLLRATH
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WILHELM ENGSTRÖM
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ARMANDO FERNANDEZ-ARIAS
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Abstract

Spider silk possesses extraordinary and unsurpassed mechanical properties and several attempts have been made to artificially produce spider silk in order to manufacture strong and light engineering composites. In the field of oncology, recombinant spider silk has the potential to be used as a biomaterial for bone replacement after tumour surgery. In this study, a 636-base pair gene fragment, coding for a part of major ampullate spidroin 1 from the African spider, Euprosthenops sp., was cloned into the expression vector pSecTag2/Hygro A, designed for the production of protein in mammalian cells. COS-1 cells were subsequently transfected with the recombinant plasmids and transient expression of low amounts of the corresponding silk protein fragment was obtained. The expressed fragment contained repetitive sequences associated with intrinsic biomechanical properties and has potential as a starting material for designed biopolymers.

Keywords:
  • Spidroin 1 gene
  • Euprosthenops
  • mammalian cells
  • spider silk protein

Footnotes

    • Received January 15, 2006.
    • Accepted February 14, 2006.
  • Copyright© 2006 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved
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Cancer Genomics - Proteomics: 3 (2)
Cancer Genomics & Proteomics
Vol. 3, Issue 2
March-April 2006
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Transient Expression of a Major Ampullate Spidroin 1 Gene Fragment from Euprosthenops sp. in Mammalian Cells
STEFAN GRIP, ANNA RISING, HELENA NIMMERVOLL, ERICA STORCKENFELDT, SIMON J. MCQUEEN-MASON, NATALIA POUCHKINA-STANTCHEVA, FRITZ VOLLRATH, WILHELM ENGSTRÖM, ARMANDO FERNANDEZ-ARIAS
Cancer Genomics & Proteomics Mar 2006, 3 (2) 83-87;

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Transient Expression of a Major Ampullate Spidroin 1 Gene Fragment from Euprosthenops sp. in Mammalian Cells
STEFAN GRIP, ANNA RISING, HELENA NIMMERVOLL, ERICA STORCKENFELDT, SIMON J. MCQUEEN-MASON, NATALIA POUCHKINA-STANTCHEVA, FRITZ VOLLRATH, WILHELM ENGSTRÖM, ARMANDO FERNANDEZ-ARIAS
Cancer Genomics & Proteomics Mar 2006, 3 (2) 83-87;
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