Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • Cancer Genomics & Proteomics
    • Anticancer Research
    • In Vivo

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Genomics & Proteomics
  • Other Publications
    • Cancer Genomics & Proteomics
    • Anticancer Research
    • In Vivo
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Cancer Genomics & Proteomics

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • In Vivo
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Visit iiar on Facebook
  • Follow us on Linkedin
Research Article

Cytoplasmic Localization of Cyclin Kinase Inhibitor p21 Delays the Progression of Apoptosis

DAISUKE ARAI, NORIHIRO NOMURA, KUNIHIKO FUKUCHI and KUNIHIDE GOMI
Cancer Genomics & Proteomics January 2006, 3 (1) 29-37;
DAISUKE ARAI
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NORIHIRO NOMURA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
KUNIHIKO FUKUCHI
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kfukuchi@med.showa-u.ac.jp
KUNIHIDE GOMI
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

The different functions of the cyclin kinase inhibitor, p21, rely on its localization to either the cytoplasm or nucleus. Phosphorylation at Thr-145 and/or Ser-146 was reported to target p21 to the cytoplasm. To clarify the function of cytoplasmic p21, we constructed non-phosphorylatable mutants, Thr-145 to Ala (T145A) and Ser-146 to Ala (S146A), and phosphorylation mimic mutants, Thr-145 to Asp (T145D) and Ser-146 to Asp (S146D), and the cells stably expressing those mutants were identified. The association of all four mutants with either CyclinA or CDK2 was increased by Á-irradiation, indicating that the mutants functioned as cyclin kinase inhibitors. PCNA binding was detected in T145A and S146A, but not in T145D and S146D. In the stably-expressing cells, T145D and S146D binding was observed in the cytoplasm, while T145A and S146A in the nucleus. Further, lactacystin treatment enhanced T145A and S146A, but not T145D and S146D, which is consistent with the degradation of p21 by proteasome in the nucleus. Apoptosis induced by Á-irradiation was delayed in the cells expressing either T145D or S146D. The activities of caspase 3 were not reduced in mutant-expressing cells. These results suggest that the PCNA-unbound form of the full length p21 in the cytoplasm delays apoptosis through the interaction with caspase 3 or downstream components.

Keywords:
  • Cyclin kinase inhibitor p21
  • apoptosis
  • DNA damage
  • Á-irradiation
  • site-directed mutant

Footnotes

    • Received November 14, 2005.
    • Revision received December 13, 2005.
    • Accepted December 20, 2005.
  • Copyright© 2006 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved
PreviousNext
Back to top

In this issue

Cancer Genomics - Proteomics: 3 (1)
Cancer Genomics & Proteomics
Vol. 3, Issue 1
January-February 2006
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Ed Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Cancer Genomics & Proteomics.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Cytoplasmic Localization of Cyclin Kinase Inhibitor p21 Delays the Progression of Apoptosis
(Your Name) has sent you a message from Cancer Genomics & Proteomics
(Your Name) thought you would like to see the Cancer Genomics & Proteomics web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
10 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Cytoplasmic Localization of Cyclin Kinase Inhibitor p21 Delays the Progression of Apoptosis
DAISUKE ARAI, NORIHIRO NOMURA, KUNIHIKO FUKUCHI, KUNIHIDE GOMI
Cancer Genomics & Proteomics Jan 2006, 3 (1) 29-37;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Cytoplasmic Localization of Cyclin Kinase Inhibitor p21 Delays the Progression of Apoptosis
DAISUKE ARAI, NORIHIRO NOMURA, KUNIHIKO FUKUCHI, KUNIHIDE GOMI
Cancer Genomics & Proteomics Jan 2006, 3 (1) 29-37;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

Similar Articles

Cancer & Genome Proteomics

© 2022 Cancer Genomics & Proteomics

Powered by HighWire