Extensive DNA Damage and Loss of Cell Viability Occur Synergistically With the Combination of Recombinant Methioninase and Paclitaxel on Pancreatic Cancer Cells which Report DNA-Damage Response in Real Time

Effect of paclitaxel alone, recombinant methioninase (rMETase) alone and their combination on DNA damage in MiaPaCa-2^Tet-On 53BP1-GFP cells. Cells were untreated (Control) or treated with 5.1 nM (IC₅₀) paclitaxel alone, 2.3 U/ml (IC₅₀) rMETase alone or their combination. (A) Images of DNA damage and repair in MiaPaCa-2^Tet-On 53BP1-GFP cells expressing green fluorescent protein (GFP). Scale bars: 100 μm. (B) Quantification of DNA damage in treated cells. GFP fluorescence was observed with an IX71 fluorescence microscope (Olympus, Tokyo, Japan). *p<0.05 (n=6) using Tukey-Kramer analysis.