Article Figures & Data
Figures
- Figure 1.
Schematic of the procedure for measuring positive staining based on our in-house developed algorithm using the Winroof imaging software. Area A was rated as the area staining positively with 4′,6-diamidino-2-phenylindole (DAPI) or antibody to cytokeratin. Area B was rated as the area staining positively for mutation-specific peptide nucleic acid (PNA)-DNA probes. Finally, the ratio of B to A was calculated. The difference in the ratio between DAPI staining and anti-cytokeratin antibody staining was statistically evaluated.
- Figure 2.
Comparison of mutation-specific peptide nucleic acid (PNA)-positive rate among epidermal growth factor receptor (EGFR) mutation-positive lung cancer tissues in 4′,6-diamidino-2-phenylindole (DAPI)-positive area. Tissues from tumors developing from four non-small-cell lung cancer cell lines were stained with each EGFR mutation-specific PNA probe and DAPI. The PNA-positive rate in DAPI-stained cancer tissues was determined. The positive rate of staining for each PNA probe in three EGFR mutation-positive lung cancer tissues was compared with that in A549 tumors without EGFR mutation and statistically evaluated using the Mann–Whitney U-test. Statistically significantly different at: *p<0.05 and **p<0.001. Magnification: 200×. Green color in images: PNA-DNA probe; cyan color: DAPI.
- Figure 3.
Comparison of mutation-specific peptide nucleic acid (PNA)-positive rate among epidermal growth factor receptor (EGFR) mutation-positive lung cancer tissues in cytokeratin-positive area. Tissues from tumors developing from four non-small-cell lung cancer cell lines were stained with each EGFR mutation-specific PNA probe and anti-cytokeratin. The PNA-positive rate in cytokeratin-stained cancer tissues was determined. The positive rate of staining for each PNA probe in three EGFR mutation-positive lung cancer tissues was compared with that in A549 tumors without EGFR mutations and statistically evaluated by the Mann-Whitney U-test. **Statistically significantly different at p<0.001. Magnification: 200×. Green color in images: PNA-DNA probe; orange color: cytokeratin.
- Figure 4.
Comparison of the cytokeratin-positive rate among non-small-cell lung cancer cell lines in the peptide nucleic acid (PNA)-DNA probe and cytokeratin staining experiments. Each panel shows the cytokeratin-positive rate in four lung cancer tissues stained with each PNA-DNA probe. Each column shows the mean±standard deviation from images of 10 areas in the stained slides. The positive rate of cytokeratin staining was compared among tissues from tumors developing from four non-small-cell lung cancer cell lines using the Mann-Whitney U-test. Magnification: 200×.
- Figure 5.
Staining with peptide nucleic acid (PNA)-DNA probe in normal human lung tissues and comparison of PNA-DNA probe staining with epidermal growth factor receptor (EGFR) mutation-specific antibody (Ab) staining in four lung cancer tissues. A: Images of hematoxylin and eosin (HE) staining and EGFR mutation-specific PNA probe staining in normal human lung tissues. Magnification: 200×. B: Comparison of EGFR mutation (L858R)-specific PNA probe staining of non-small-cell lung cancer tissues with EGFR L858R monoclonal antibody staining. Green color in images: PNA-DNA probe; orange color: cytokeratin. Magnification: 200×.
- Figure 6.
Correlation analysis of the positive rates of peptide nucleic acid (PNA)-DNA probe staining and epidermal growth factor receptor (EGFR) mutation (L858R)-specific antibody staining in NCI-H1975 tumor tissue. The positive rates of PNA-DNA probe and anti-EGFR L858R from 10 images were plotted. Correlation between the staining ability of the PNA-DNA probe and anti-EGFR L858R was statistically evaluated using Pearson correlation analysis. Magnification: 200×.
Tables
In this issue
Jump to section
Related Articles
Cited By...
- No citing articles found.