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Localization of EGFR Mutations in Non-small-cell Lung Cancer Tissues Using Mutation-specific PNA-DNA Probes

HAJIME SHIGETO, HARUO MIYATA, TADASHI ASHIZAWA, AKIRA IIZUKA, YASUFUMI KIKUCHI, CHIKAKO HOZUMI, CHIE MAEDA, KEN YAMAGUCHI, SHOHEI YAMAMURA and YASUTO AKIYAMA
Cancer Genomics & Proteomics July 2023, 20 (4) 375-382; DOI: https://doi.org/10.21873/cgp.20389
HAJIME SHIGETO
1Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Kagawa, Japan;
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HARUO MIYATA
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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TADASHI ASHIZAWA
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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AKIRA IIZUKA
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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YASUFUMI KIKUCHI
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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CHIKAKO HOZUMI
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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CHIE MAEDA
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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KEN YAMAGUCHI
3Shizuoka Cancer Center, Shizuoka, Japan
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SHOHEI YAMAMURA
1Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Kagawa, Japan;
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YASUTO AKIYAMA
2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
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  • For correspondence: y.akiyama{at}scchr.jp
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Article Information

vol. 20 no. 4 375-382
DOI 
https://doi.org/10.21873/cgp.20389
PMID 
37400147

Published By 
International Institute of Anticancer Research
Print ISSN 
1109-6535
Online ISSN 
1790-6245
History 
  • Received April 11, 2023
  • Revision received June 9, 2023
  • Accepted June 19, 2023
  • Published online July 3, 2023.

Copyright & License 
Copyright © 2023 The Author(s). Published by the International Institute of Anticancer Research. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY-NC-ND) 4.0 international license (https://creativecommons.org/licenses/by-nc-nd/4.0).

Author Information

  1. HAJIME SHIGETO1,*,
  2. HARUO MIYATA2,*,
  3. TADASHI ASHIZAWA2,
  4. AKIRA IIZUKA2,
  5. YASUFUMI KIKUCHI2,
  6. CHIKAKO HOZUMI2,
  7. CHIE MAEDA2,
  8. KEN YAMAGUCHI3,
  9. SHOHEI YAMAMURA1 and
  10. YASUTO AKIYAMA2⇑
  1. 1Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Kagawa, Japan;
  2. 2Immunotherapy Division, Shizuoka Cancer Center Research Institute, Shizuoka, Japan;
  3. 3Shizuoka Cancer Center, Shizuoka, Japan
  1. Correspondence to: Yasuto Akiyama, MD, Immunotherapy Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka 411-8777, Japan. Tel.: +81 559895222 (Ext. 5330), Fax: +81 559896085, e-mail: y.akiyama{at}scchr.jp
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Cancer Genomics - Proteomics: 20 (4)
Cancer Genomics & Proteomics
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Localization of EGFR Mutations in Non-small-cell Lung Cancer Tissues Using Mutation-specific PNA-DNA Probes
HAJIME SHIGETO, HARUO MIYATA, TADASHI ASHIZAWA, AKIRA IIZUKA, YASUFUMI KIKUCHI, CHIKAKO HOZUMI, CHIE MAEDA, KEN YAMAGUCHI, SHOHEI YAMAMURA, YASUTO AKIYAMA
Cancer Genomics & Proteomics Jul 2023, 20 (4) 375-382; DOI: 10.21873/cgp.20389

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Localization of EGFR Mutations in Non-small-cell Lung Cancer Tissues Using Mutation-specific PNA-DNA Probes
HAJIME SHIGETO, HARUO MIYATA, TADASHI ASHIZAWA, AKIRA IIZUKA, YASUFUMI KIKUCHI, CHIKAKO HOZUMI, CHIE MAEDA, KEN YAMAGUCHI, SHOHEI YAMAMURA, YASUTO AKIYAMA
Cancer Genomics & Proteomics Jul 2023, 20 (4) 375-382; DOI: 10.21873/cgp.20389
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Keywords

  • PNA-DNA probe
  • EGFR mutation
  • tumor heterogeneity
  • multicolor immunofluorescence
  • image analysis software
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