Article Figures & Data
Figures
- Figure 1.
Analysis of ANROC expression. A: A schematic diagram of ANROC, ANRIL, p16INK4A, p15INK4B, and ARF. The arrow heads indicate the direction of transcription. The black boxes show the exons of each gene. B: ANROC expression in the indicated human cell lines was detected by RT-PCR. Ribosomal protein L32 (RPL32) expression was detected as an internal control. C: HeLa cells were infected with retroviruses carrying HRASG12V or mock vector. The expression levels of ANROC were assessed by qRT-PCR. The results are shown as relative values based on the values of HeLa cells/mock. *p<0.05.
- Figure 2.
Silencing of ANROC promotes HeLa cell proliferation. A: Phase-contrast images of HeLa cells transfected with control siRNA (Ctr-i) or siRNA against ANROC (ANROC-i) for 72 h. B: Proliferation curves of HeLa cells/Ctr-i and /ANROC-i. Viable cells were counted by staining with trypan blue. The x-axis indicates days since transfection of siRNA. C: CAL27 cells transfected with siRNA for 72 h were counted by trypan blue staining. *p<0.05.
- Figure 3.
Silencing of ANROC increases the mRNA expression levels of p16INK4A, p15INK4B, ARF, and cyclin B1. The mRNA levels of ANROC, p16INK4A, p15INK4B, ARF, ANRIL (A) and cyclins (B) were measured by qRT-PCR. The results are shown as relative values based on the values of HeLa cells transfected with control siRNA (Ctr-i). *p<0.05, **p<0.01, n.s., not significant.
- Figure 4.
Silencing of ANROC results in an increase in the number of cells in the S and G2/M phases and a decrease in the number of cells in the G1 phase. A: Histogram of cell cycle analysis of HeLa cells transfected with control siRNA (Ctr-i) or siRNA against ANROC (ANROC-i) for 72 h. The x-axes show the fluorescence intensity of propidium iodide (DNA content index). The y-axes show cell number. B: The percentages of HeLa cells transfected with siRNA in the G1, S, and G2/M phase. ***p<0.001.
- Figure 5.
Model of ANROC function. ANROC transcribed from the INK4 locus suppresses the expression of p16INK4A, p15INK4B, ARF, and cyclin B1 in cis- and trans-acting manners. ANROC suppresses the progression of the cell cycle, leading to the inhibition of cell proliferation.
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