Prognostic Significance of Transient Receptor Potential Vanilloid Type 1 (TRPV1) and Phosphatase and Tension Homolog (PTEN) in Epithelial Ovarian Cancer

GWAN HEE HAN; DOO BYUNG CHAY; SANGHEE NAM; HANBYOUL CHO; JOON-YONG CHUNG; JAE-HOON KIM

doi:10.21873/cgp.20191

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Figure 1.
TRPV1 and PTEN expression in formalin-embedded non-adjacent normal epithelium, benign tumor, borderline tumor, and epithelial ovarian cancer tissues. (A) Representative immunohistochemical images of TRPV1 and PTEN in non-adjacent normal epithelium, benign tumor, borderline tumor, and epithelial ovarian cancer tissues. Scale bar: 60 μm. (B) IHC staining score for TRPV1 and PTEN in nonadjacent normal epithelium, benign tumor, borderline tumor, and epithelial ovarian cancer tissues.
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Figure 2.
Spearman's rank correlation analysis between TRPV1 and PTEN expression. TRPV1 expression showed a trend of negative correlation with PTEN level in EOCs (A), while in stage III/IV, there was a significant negative correlation between TRPV1 and PTEN (B). TRPV1−/PTEN+ was significantly associated with FIGO stage (C) and serous cell type (D).
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Figure 3.
Kaplan–Meier survival curves for TRPV1 and PTEN expression in EOC. EOC patients with high TRPV1 expression had a shorter overall survival (A) (p<0.001) and worse 5-year disease-free survival (B) (p<0.001) than those with low TRPV1 expression. The patients with high expression of PTEN showed a longer 5-year disease-free survival (D) (p=0.026) and those with high TRPV1/low PTEN had a shorter overall survival (E) (p=0.021) and worse 5-year disease-free survival (F) (p<0.001) than patients with low TRPV1/high PTEN expression.
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Figure 4.
Effect of TRPV1 overexpression on EOC cells. Relative mRNA levels in control and TRPV1-knockdown A2780 cells (A). Viability curves for A2780 cells transfected with sh-control, sh-TRPV1 knockdown (B). A2780 cells were collected at various time points. The viability of TRPV1 knockdown cells was decreased. Quantification of cell counting assay for sh-control and sh-TRPV1-transfected A2980 cells (C). It showed significantly decreased cell number in sh-TRPV1 transfected A2980 cells compared to sh-control. The colony formation assay of sh-control and sh-TRPV1 transfected A2980 cells (D) Left panel: Representative images of colonogenic assay; Right panel: Quantitative results of colonogenic assay. Colony formation ability was decreased for the TRPV1 knockdown cells as compared with the control cells. The number of asterisks (*) indicates the level of significance: *p≤0.05. Data and error bars represent the mean±SD of triplicate experiments.