Article Figures & Data
Figures
- Figure 1.
Clinical significance of KIF15 expression in HCC tissues. (A) KIF15 mRNA expression in tumor tissues and non-cancerous tissues in our HCC patient cohort, TCGA dataset and GSE14520 dataset. (B) Immunohistochemical detection of KIF15 expression in a representative sample of HCC. Original magnifications: ×50, ×200 and ×400. (C) KIF15 mRNA expression in HCC cell lines (HepG2 and HuH7) and monocyte and fibroblast cell lines (THP1 and KMST-6, respectively). (D) Kaplan-Meier survival curve of 59 HCC patients from our patient cohort, 335 HCC patients from a TCGA dataset, and 242 HCC patients from the GSE14520 dataset based on KIF15 mRNA expression. (E) GSEA of HCC cases from a TCGA dataset.
- Figure 2.
HCC cells with a high expression of MCM2 were located close to inflammatory monocytes that expressed KIF15. (A) Immunofluorescence images of KIF15 and MCM2 expression in HCC tissues. (B) Correlation between the number of inflammatory monocytes that expressed high levels of KIF15 and HCC cells that expressed high levels of MCM2 in HCC tissues. (C) Correlation between KIF15 mRNA expression and MCM2 or PCNA mRNA expression in the TCGA dataset.
- Figure 3.
KIF15-expressing PBMC promoted HCC cell proliferation in vitro. Coculture of HepG2 and HuH7 cells and healthy donor PBMCs. (A) Immunohistochemical detection of KIF15 expression in PBMC and HepG2 cells. Original magnification, ×200. (B) Colony proliferation assay after co-culture of HepG2 or HuH7 cells with healthy donor PBMC. (C) MTT assay after co-culture of HepG2 cells with healthy donor PBMC. (D) Western blot analysis for MCM2 expression in HepG2 cells co-cultured with healthy donor PBMC.
