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Research Article
Open Access

MiR-376b-3p Is Associated With Long-term Response to Sunitinib in Metastatic Renal Cell Carcinoma Patients

JULIA KOVACOVA, JAROSLAV JURACEK, ALEXANDR POPRACH, JINDRICH KOPECKY, ONDREJ FIALA, MAREK SVOBODA, PAVEL FABIAN, LENKA RADOVA, PETR BRABEC, TOMAS BUCHLER and ONDREJ SLABY
Cancer Genomics & Proteomics September 2019, 16 (5) 353-359; DOI: https://doi.org/10.21873/cgp.20140
JULIA KOVACOVA
1Masaryk University, Central European Institute of Technology, Brno, Czech Republic
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JAROSLAV JURACEK
1Masaryk University, Central European Institute of Technology, Brno, Czech Republic
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ALEXANDR POPRACH
2Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk University, Brno, Czech Republic
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JINDRICH KOPECKY
3Department of Clinical Oncology and Radiotherapy, University Hospital in Hradec Kralove, Hradec Kralove, Czech Republic
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ONDREJ FIALA
4Department of Oncology and Radiotherapeutics, Medical School and Teaching Hospital in Pilsen, Charles University, Pilsen, Czech Republic
5Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic
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MAREK SVOBODA
2Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk University, Brno, Czech Republic
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PAVEL FABIAN
6Masaryk Memorial Cancer Institute, Department of Oncological and Experimental Pathology Faculty of Medicine, Masaryk University, Brno, Czech Republic
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LENKA RADOVA
1Masaryk University, Central European Institute of Technology, Brno, Czech Republic
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PETR BRABEC
7Institute for Biostatistics and Analyses, Masaryk University, Brno, Czech Republic
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TOMAS BUCHLER
8Department of Oncology, First Faculty of Medicine, Charles University and Thomayer Hospital, Prague, Czech Republic
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ONDREJ SLABY
2Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Faculty of Medicine, Masaryk University, Brno, Czech Republic
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  • For correspondence: on.slaby{at}gmail.com
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    Figure 1.

    Design of the study. FFPE: Formalin-fixed paraffin embedded; RT-qPCR: reverse transcription real time quantitative polymerase chain reaction.

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    Figure 2.

    MiR-376b-3p expression levels in tumors of patients with primary resistance (PFS=2-5 months, N=30), intermediate response (PFS 5-12 months, N=42) and long-term response to sunitinib therapy (PFS >12 months, N=42). (Kruskall–Wallis test; p=0.0003). Comparison only between patients with primary resistance and long-term response (Mann–Whitney test; ***p=0.0001).

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    Figure 3.

    Receiver operating characteristic analysis of the miR-376b-3p as a discriminator between patients with primary resistance and long-term response to sunitinib treatment (p=0.0002).

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    Figure 4.

    Survival curves of patients stratified accordingly to miR-376b-3p expression level (cut-off value=0.0244; p<0.0001).

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Cancer Genomics & Proteomics
Vol. 16, Issue 5
September-October 2019
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MiR-376b-3p Is Associated With Long-term Response to Sunitinib in Metastatic Renal Cell Carcinoma Patients
JULIA KOVACOVA, JAROSLAV JURACEK, ALEXANDR POPRACH, JINDRICH KOPECKY, ONDREJ FIALA, MAREK SVOBODA, PAVEL FABIAN, LENKA RADOVA, PETR BRABEC, TOMAS BUCHLER, ONDREJ SLABY
Cancer Genomics & Proteomics Sep 2019, 16 (5) 353-359; DOI: 10.21873/cgp.20140

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MiR-376b-3p Is Associated With Long-term Response to Sunitinib in Metastatic Renal Cell Carcinoma Patients
JULIA KOVACOVA, JAROSLAV JURACEK, ALEXANDR POPRACH, JINDRICH KOPECKY, ONDREJ FIALA, MAREK SVOBODA, PAVEL FABIAN, LENKA RADOVA, PETR BRABEC, TOMAS BUCHLER, ONDREJ SLABY
Cancer Genomics & Proteomics Sep 2019, 16 (5) 353-359; DOI: 10.21873/cgp.20140
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Keywords

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