Article Figures & Data
Figures
- Figure 1.
Overview of major steps of identification protein–protein interactions using four methods compared in this study (Methods 1-4). Individual methods differed in (i) solid supports for tagged protein capture (streptavidin agarose beads, SPR chip), (ii) mechanics of the washing and elution steps (centrifugation, gravity flow, microflow) and (iii) type and concentration of detergents (detailed in Table I). Protein quantification was performed using SWATH-MS for all methods.
- Figure 2.
Typical sensorgram from interaction of the lysate with streptavidin-modified SPR chip and elution of the captured proteins using the MICRORECOVERY procedure of Biacore.
- Figure 3.
Verification of successful incorporation of pLENTI-N-SBP-PDLIM2-IRES-GFP and pLENTI-N-SBP-GFP vectors in MCF7 cells. A) Detection of PDLIM2 protein levels in non-transfected MCF7 cells and stably transfected MCF7 N-SBP-PDLIM2 cells and MCF7 N-SBP-GFP cells. B) Detection of Streptavidin-binding peptide in non-transfected MCF7 cells and stably-transfected MCF7 N-SBP-PDLIM2 cells and MCF7 N-SBP-GFP cells. Proliferating cell nuclear antigen (PCNA) was used as a loading control.
