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Research Article
Open Access

Pull-down Assay on Streptavidin Beads and Surface Plasmon Resonance Chips for SWATH-MS-based Interactomics

JOSEF MARYÁŠ, JAKUB FAKTOR, LENKA ČÁPKOVÁ, PETR MÜLLER, PETR SKLÁDAL and PAVEL BOUCHAL
Cancer Genomics & Proteomics September 2018, 15 (5) 395-404; DOI: https://doi.org/10.21873/cgp.20098
JOSEF MARYÁŠ
1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
2Masaryk Memorial Cancer Institute, Regional Centre for Applied Molecular Oncology, Brno, Czech Republic
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JAKUB FAKTOR
1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
2Masaryk Memorial Cancer Institute, Regional Centre for Applied Molecular Oncology, Brno, Czech Republic
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LENKA ČÁPKOVÁ
1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
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PETR MÜLLER
2Masaryk Memorial Cancer Institute, Regional Centre for Applied Molecular Oncology, Brno, Czech Republic
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PETR SKLÁDAL
1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
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PAVEL BOUCHAL
1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
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  • For correspondence: bouchal{at}chemi.muni.cz
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Article Information

vol. 15 no. 5 395-404
DOI 
https://doi.org/10.21873/cgp.20098
PMID 
30194080

Published By 
International Institute of Anticancer Research
Print ISSN 
1109-6535
Online ISSN 
1790-6245
History 
  • Received July 4, 2018
  • Revision received July 26, 2018
  • Accepted July 27, 2018
  • Published online September 7, 2018.

Copyright & License 
Copyright © 2018 The Author(s). Published by the International Institute of Anticancer Research.

Author Information

  1. JOSEF MARYÁŠ1,2,
  2. JAKUB FAKTOR1,2,
  3. LENKA ČÁPKOVÁ1,
  4. PETR MÜLLER2,
  5. PETR SKLÁDAL1 and
  6. PAVEL BOUCHAL1⇑
  1. 1Masaryk University, Faculty of Science, Department of Biochemistry, Brno, Czech Republic
  2. 2Masaryk Memorial Cancer Institute, Regional Centre for Applied Molecular Oncology, Brno, Czech Republic
  1. Correspondence to: Pavel Bouchal, Ph.D., Masaryk University, Faculty of Science, Department of Biochemistry, Kotlarska 2, 61137 Brno, Czech Republic. Tel: +420 549493251, Fax: +420 549492690, e-mail: bouchal{at}chemi.muni.cz
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Cancer Genomics & Proteomics
Vol. 15, Issue 5
September-October 2018
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Pull-down Assay on Streptavidin Beads and Surface Plasmon Resonance Chips for SWATH-MS-based Interactomics
JOSEF MARYÁŠ, JAKUB FAKTOR, LENKA ČÁPKOVÁ, PETR MÜLLER, PETR SKLÁDAL, PAVEL BOUCHAL
Cancer Genomics & Proteomics Sep 2018, 15 (5) 395-404; DOI: 10.21873/cgp.20098

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Pull-down Assay on Streptavidin Beads and Surface Plasmon Resonance Chips for SWATH-MS-based Interactomics
JOSEF MARYÁŠ, JAKUB FAKTOR, LENKA ČÁPKOVÁ, PETR MÜLLER, PETR SKLÁDAL, PAVEL BOUCHAL
Cancer Genomics & Proteomics Sep 2018, 15 (5) 395-404; DOI: 10.21873/cgp.20098
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Keywords

  • LC-SWATH-MS/MS
  • pull-down assay
  • PDLIM2
  • protein-protein interactions
  • SPR
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