Article Figures & Data
Figures
- Figure 1.
Scatter plot of gene expression level determined by the DASL assay and RNA sequencing. RNA expression profiles for samples N11 and P08 are demonstrated in this Figure. Based on the results of the DASL assay, all genes were separated into three groups: the high-expression, medium-expression, and low-expression group. The quantile-normalized gene expression level derived from DASL assay (QTLDASL) and RNA-sequencing (QTLRSEQ) for each group was plotted. The Pearson's correlation coefficient between the two platforms in each group was shown on the top of each plot.
- Figure 2.
Correlation of the expression data determined by the DASL assay and RNA sequencing. The Pearson's correlation coefficients for genes with different expression levels in each sample were plotted.
- Figure 3.
Correlation of the pathway signatures derived from the two platforms. The pathways or gene sets that were enriched in the expression data derived from the two platforms were analyzed by GSEA. The normalized enrichment score (NES) of each pathway in both platforms was plotted.
- Figure 4.
Correlation of the expression level determined by RT-qPCR and each platform. Expression levels of 12 genes were determined by RT-qPCR to confirm the data derived from the DASL and RSEQ assays. A, The expression level of each gene determined by both platforms and the corresponding CT value determined by RT-qPCR. B, The summary of Pearson's correlation coefficients between RT-qPCR and each platform in all patients is shown.
