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Isolation of Stem Cells Using Spheroids from Fresh Surgical Specimen: An Analytic Mini-Review

ITZHAK AVITAL, ALEXANDER STOJADINOVIC, HONGJIAN WANG, CIARAN MANNION, WILLIAMS CS CHO, JINLIAN WANG and YAN GAO MAN
Cancer Genomics & Proteomics March 2014, 11 (2) 57-65;
ITZHAK AVITAL
1 Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.
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ALEXANDER STOJADINOVIC
1 Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.
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HONGJIAN WANG
2 College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou, P.R. China
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CIARAN MANNION
3 Department of Pathology, Hackensack University Medical Center, Hackensack, NJ, U.S.A.
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WILLIAMS CS CHO
4 Department of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, P.R. China
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JINLIAN WANG
5 Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, U.S.A.
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YAN GAO MAN
1 Bon Secours Cancer Institute, Bon Secours Health System, Richmond, VA, U.S.A.
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    Figure 1.

    Main steps for isolation of stem cells from human hepatoma cell lines and resected liver cancer samples.

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    Figure 2.

    Main steps for isolation of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens.

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    Figure 3.

    Main steps for isolation of label-retaining cancer cells from human gastrointestinal cancer.

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    Figure 4.

    Main steps for isolation of adult stem/progenitor cells from human thyroid.

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    Figure 5.

    Main steps for isolation of adult breast stem/progenitor cells from surgical specimens.

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    Figure 6.

    Focal capsule disruptions in normal-appearing epithelial structures. Normal-appearing prostate and breast tissue sections were double-immunostained for capsule (thick arrows), lymphocytes (thin arrows), and estrogen receptor (brown). Circles identify focal capsule disruptions. Note that several lymphocytes infiltrate the epithelial cells overlying focal capsule disruption. Also note that epithelial cells overlying focal capsule disruptions lack ER expression. Original magnification, ×150.

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Cancer Genomics & Proteomics
Vol. 11, Issue 2
March-April 2014
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Isolation of Stem Cells Using Spheroids from Fresh Surgical Specimen: An Analytic Mini-Review
ITZHAK AVITAL, ALEXANDER STOJADINOVIC, HONGJIAN WANG, CIARAN MANNION, WILLIAMS CS CHO, JINLIAN WANG, YAN GAO MAN
Cancer Genomics & Proteomics Mar 2014, 11 (2) 57-65;

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Isolation of Stem Cells Using Spheroids from Fresh Surgical Specimen: An Analytic Mini-Review
ITZHAK AVITAL, ALEXANDER STOJADINOVIC, HONGJIAN WANG, CIARAN MANNION, WILLIAMS CS CHO, JINLIAN WANG, YAN GAO MAN
Cancer Genomics & Proteomics Mar 2014, 11 (2) 57-65;
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    • Abstract
    • Major Technical Steps and Primary Findings
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Keywords

  • Adult stem cells
  • cellular replenishment
  • carcinogenesis
  • spheroid
  • surgical specimens
  • review
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