Abstract
Aim: This study examined the expression of PLGF-1 and PGF-2 in human colorectal cancer and how changes in expression may be linked to prognosis of the disease. Materials and Methods: Expression of PLGF transcripts in primary colorectal tumours (n=94) and background tissue (n=80) were analysed by Q-PCR. Immunohistochemistry was carried out on a number of matched background and tumour sections. Results: There was a progressive increase in Dukes A to C (430±134 versus 674±2680) and with TNM 1 to 3 as compared to background tissues (479±152 versus 777±327 p=0.032). Interestingly, there was an increase in PLGF-1 expression in patients with poor outcome compared with patients remaining disease-free. PGF-2 expression was significantly elevated in tumours compared to normal (4558±240 versus 859±67.3 p<0.0001). Moreover, expression was increased in patients with poor prognosis compared to those remaining disease free (4585±285 versus 3909±446). Conclusion: There is increased expression of the PLGF isoforms in human colorectal cancer.
- Colorectal cancer
- immunohistochemistry
- placenta growth factor
- prognosis
- real-time quantitative polymerase chain reaction
- vascular endothelial growth factor
Footnotes
- Received June 30, 2009.
- Accepted July 7, 2009.
- Copyright© 2009 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved