Abstract
Functional complementation of cellular defects has been a valuable approach for localizing causative genes to specific chromosomes. The complementation strategy was followed by positional cloning and characterization of genes for their biological relevance. We herein describe strategies used for the construction of monochromosomal hybrids and their applications for cloning and characterization of genes related to cell growth, cell senescence and DNA repair. We have cloned RNaseT2, GluR6 (glutamate ionotropic receptor kainate type subunit 2-GRIK2) and protein tyrosine phosphatase, receptor type K (PTPRK) genes using these strategies.
- Chromosome transfer
- monochromosomal hybrids
- functional cloning
- cell senescence
- senescence associated β-galactosidase
- glutamate ionotropic receptor kainate type subunit 2
- GRIK-2
- GluR6
- protein tyrosine phosphatase
- receptor type K (PTPRK)
- review
- Received January 9, 2017.
- Revision received February 19, 2017.
- Accepted February 22, 2017.
- Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved