Abstract
Background: The precise mechanism of the therapeutic effects of fucoidan (sulphated polyfucose) on cultured hepatocarcinoma HepG2 cells is as yet unclear. Materials and Methods: Protein components between fucoidan-treated and non-treated HepG2 cells were compared through a quantitative micro-sequencing method. Results: A dramatic and immediate increment of the membrane compartment and a decrement of RNA virus by fucoidan, as an effect of the Ishi-Mozuku (an edible brown seaweed Mozuku of Japan), are demonstrated. The ratio of membrane glycoproteins to total cellular proteins increases from 28.9% to 43.2% (1.5-fold), and the positive-sense single-stranded RNA viral proteins among the total cellular proteins decrease from 5.3% to 0.29% (18-fold), respectively, in response to 0.102 mg/ml fucoidan in HepG2 cells over three days’ period. Conclusion: Fucoidan seems to retard the growth of HepG2 cells through membrane glycoprotein metabolism. Therefore, fucoidan could be expected to have a therapeutic effect on hepatocellular carcinoma.
- Direct micro-sequencing
- proteomics
- protein determination
- invaded microbes
- membrane protein
- hydrophobicity
- de-anchoring
- liver cancer
- HCC
- HCV
- HIV
- viral proteins
- Ishi-Mozuku
- fucoidan
- Received November 24, 2013.
- Revision received February 21, 2014.
- Accepted February 24, 2014.
- Copyright© 2014, International Institute of Anticancer Research (Dr. John G. Delinasios), All rights reserved